Coding

Part:BBa_K4455006:Design

Designed by: Gabriella Cerna   Group: iGEM22_ASU   (2022-10-11)


Phytochelatin Synthase Optimized for Chlamydomonas Reinhardtii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 540
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1364
    Illegal PstI site found at 540
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1673
    Illegal XhoI site found at 280
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 540
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 540
    Illegal NgoMIV site found at 1885
    Illegal AgeI site found at 478
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for phytochelatin synthase was codon optimized for use in Chlamydomonas reinhardtii. Additionally, introns were inserted into the sequence to improve transgene expression.


Source

The sequence for phytochelatin synthase was derived from the genomic sequence of Arabidopsis thaliana. The original sequence prior to optimization can be found at the following accession number: Q9S7Z3.1.

References